About ChromoScreen

The Aim

Most in vitro fertilization (IVF) cycles fail because of aneuploid embryos; their elimination prior to embryo transfer will, therefore, improve IVF outcomes.
Gleicher and Orvieto, Journal of Ovarian Research (2017) 10:21

PGT/A and aCGH allow for the transfer of a single euploid embryo reducing the risks associated with multiple gestations.

Aneuploidy screening for 24 chromosomes significantly improves clinical outcomes by avoiding the transfer of aneuploid embryos. Therefore this technique is able to increase the chance for implantation and decrease the risk of miscarriages associated with aneuploid.

ChromoScreen® is the IBSA solution used in PGT/A (Preimplantation Genetic Testing for Aneuploidies) for detecting chromosomal aneuploidies in embryos from the IVF cycle before implantation. Chromoscreen kit is able to detect imbalances in any of the 24 chromosomes, aiming to identify those embryos least likely to have aneuploidy for transfer.

The test is based on array comparative genomic hybridization (aCGH) in which the DNA to test is compared to a normal control DNA on the same array. PGT/A with Chromoscreen microarrays provides a clear and accurate assessment of all 24 chromosomes from an embryo biopsy.

The Features

  • The clone library is tailored to the size and GC content of each chromosome

  • Optimal balance between human genome coverage and clear data processing

  • Screening of all 24 chromosomes in less than 24 hours

  • The design of the slide allows to analyze 4 different samples (even deriving from different patients) by using a single slide

  • Fast and reliable molecular karyotype provided by the ChromoScreen software

The Samples

ChromoScreen array is designed in order to perform the analysis using a really small quantity of DNA from a single cell; in particular this technology can potentially be performed on three different cell types during pre-implantation development:

  • polar bodies (PBs) from the oocyte

  • blastomeres (BS) from cleavage-stage embryos or

  • trophectodermal cells (TE) deriving from the blastocyst.